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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Seroprevalence of Bovine Leukemia Virus (BLV) Infection in Pregnant Replacement Dairy Heifers in Saraburi Province, Thailand) ผู้เขียน: and Sunthorn Rungruang สื่อสิ่งพิมพ์:pdf AbstractSeroprevalence of bovine leukemia virus (BLV) infection was studied in replacement dairy heifers. Blood samples were collected from 80 pregnant heifers raised in 8 dairy farms in Saraburi province, the central part of Thailand and serum samples were tested for antibodies against BLV infection using commercially available ELISA test kits. The results revealed that 26 (32.5%) pregnant heifers were positive reactors, which was higher than seroprevalence of BLV in lactating dairy cows previously reported in Thailand. This study provided an evidence of a high seroprevalence of BLV in replacement heifers raised in areas of Saraburi province. It was crucial to the dairy farmers to be aware of this disease in replacement heifer rearing. Although serological test does not provide information of the infection at the early stage and is not sensitive enough to detect every infected animals, elimination of seropositive animals and prohibition of introduction of seropositive animals are sufficient to promote BLV-free herds in Thailand. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Fatty Liver in High Producing Dairy Cows Kept in Evaporative Cooling System in a Commercial Dairy Herd in Thailand) ผู้เขียน: ดร.ธีระ รักความสุข, รองศาสตราจารย์ , Sunthorn Rungruang, Theo Wensing สื่อสิ่งพิมพ์:pdf AbstractThe occurrence of fatty liver was investigated in a commercial dairy farm under evaporative cooling system in Thailand by determination of triacylglycerol concentrations in the liver. Nineteen Holstein-Friesian cows were used. Liver samples were collected at -2, 1, 2, 3, and 4 wk from parturition. At -2 wk, mean liver triacylglycerol concentration was 24.3 ? 1.0 mg/g of liver. After parturition, mean liver triacylglycerol concentration from 6 cows was lower than 50 mg/g of liver (mild fatty liver group), the mean concentration from 8 cows was between 50 and 100 mg/g of liver (moderate fatty liver group), and the mean concentration from 5 cows was greater than 100 mg/g of liver (severe fatty liver group). The results showed that 68.4% of dairy cows in this investigation suffered from periparturient fatty liver. Milk production in severe fatty liver group tended to be higher than the milk production in mild or moderate fatty liver cows. This result indicated that cows with higher milk yield tended to enter deeper negative energy balance postpartum and as a consequence had more intensive lipolysis, leading to accumulation of a greater amount of triacylglycerols in the liver. In conclusion, an evidence of fatty liver was found in high producing dairy cows, kept under cooled condition in Thailand. Before any preventive measures could be made, further research is required to study the effect of fatty liver or negative energy balance on periparturient problems, i.e. infertility, metabolic disorders, in dairy cows raised in Thai conditions. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Relationship between the Sodium Nitroprusside Test for Ketone Bodies in Urine and Serum ?-Hydroxybutyrate Concentrations in Dairy Cows) ผู้เขียน:ดร.ธีระ รักความสุข, รองศาสตราจารย์, Jenjira Suksiri, Nonthasit Chutiyanawat, Nathakorn Kaewsakhorn, Sunthorn Rungruang สื่อสิ่งพิมพ์:pdf AbstractThe relationship between the sodium nitroprusside (SNP) test for ketone bodies in urine and concentrations of serum ?-hydroxybutyrate (?-HB) was studied in 50 Holstein Friesian cows on a commercial dairy farm, including 10 cows in the dry period, and 22, 8 and 10 cows and in week 2, 4 and 8 of lactation, respectively. Urine and blood samples were collected from all cows and were transported to the laboratory on the sampling date. Urine samples were analyzed for ketone bodies using the SNP test within 24 hours after collection. Serum samples were analyzed for the concentration of ?-HB. Results of the SNP test for ketone bodies were classified as either N (negative), +1 or + 2 based on the color reaction of SNP with a known amount of acetone in aqueous solution at 0 mmol (N), 1 to 5 mmol (+1) and 6 to 10 mmol (+2) respectively. Results of the SNP test for ketone bodies in urine were highly correlated with the concentrations of ?-HB in the serum (r = 0.82). Concentrations of serum ?-HB were 0.46 ? 0.03, 0.98 ? 0.10 and 2.63 ? 0.34 mmol/L for cows with the SNP test results of N (n = 31), +1 (n = 10) and +2 (n = 9), respectively. Concentrations of serum ?-HB were 0.42 ? 0.04, 0.97 ? 0.21, 1.48 ? 0.44 and 0.89 ? 0.12 mmol/L for cows in the dry period and in week 2, 4 and 8 of lactation, respectively. The method was practical, simple and inexpensive and therefore, it would be very beneficial for the diagnosis of subclinical ketosis or the early detection of ketosis in dairy cows, particularly on smallholder farms. |
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 Researcherดร. กานต์สุดา วันจันทึก, รองศาสตราจารย์ที่ทำงาน:ภาควิชาคหกรรมศาสตร์ คณะเกษตร สาขาที่สนใจ:Experimental nutrition and food biochemistry, Nutrition throughout the life cycle, Antioxidants and Anti-inflammation, Digital technologies for nutrition education Resume |